As shown in Figure 1, first, click on the "Home" tab at the toolbar; select the database which you want to search and can link to the database of each body fluid.
Figure 1. Home
As shown in Figure 2, first, click on the "Browse" tab at the toolbar; select the database which you want to search; then click the "Browse" button, and the protein level result of the database will be displayed. (Figure 3)
Figure 2. browse the database
The protein level result includes 9 columns (Figure 3). The first column shows the Swiss-Prot accession number of each protein, which also provides the hyperlink that enable you to browse the protein information in the Swiss-Prot database. The second column shows the protein name in the Swiss-Prot database. The third column shows the molecular weight of the protein. The fourth column shows the pI value of the protein. The fifth column shows the estimated concentration of the protein in plasma. The sixth column shows the estimated concentration of the protein in CSF.The seventh column shows the estimated concentration of the protein in saliva.The eighth column shows the estimated concentration of the protein in urine.The ninth column shows the estimated concentration of the protein in AF.In addition, you can filter the resulting dataset by input in any data inside the table to the search textbox, and sorting the dataset by clicking each column head. By clicking the abundance number , users can easily entered the plasma database and more detailed information of the protein in plasma was displayed.
Figure 3. browse the protein level data
You can search the database by protein accession (only support the Swiss-Prot protein accession) or peptide sequence (Figure 4). First you select "protein" and input the Swiss-Prot protein accession in the text box, or select "peptide" and input the middle sequence of the peptide (not include in the first amino acid and the last amino acid). Second, you select the display level of the search result, which includes protein level or peptide level as described before. Third, you select the database which you search. Then click "Query" button, and the result will be shown.
Figure 4. search the database